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  ETHOLOGY AND ITS METHODS   The various aspects related with animal behavior can be studied by applying the following methods - 1. Under natural conditions. 2. Experimental Analysis 3. Statistical Analysis 1. Under Natural Conditions: - The best way of studying animal behavior is under natural conditions. Observation may be in the form of field notes, checklist of behavior patterns, tape recorders, motion pictures etc. While studying animal behavior in natural surroundings circadian rhythms and seasonal cycles are taken into consideration. 2. Experimental Analysis: - Experiments are conducted in two ways viz. Field experiment and Laboratory experiment – (A) Field Experiment: - These are performed with least disturbance to the animal. The first phase involves a thorough description of all aspects of the animal under observation. For this binocular and infrared devices are required when more than one individuals are to be observed, it is desirable to make...

LAC CULTURE || ECONOMIC ZOOLOGY || B.Sc Class Notes|| PDF

LAC CULTURE

Lac is a natural resin of animal origin. It is secreted by an insect, known as lac-insect. In order to obtain lac, these insects are cultured and the technique is called lac-culture. It involves proper care of host plants, regular pruning of host plants, propagation, collection and processing of lac.

 

History and Nomenclature

 

Lac has been used in India from time immemorial for several purposes, from the epic of Mahabharat it has been recorded that Kauravas built a palace of lac for the destruction of Pandavas. We come across references of lac in the Atharvaveda and Mahabharata, so it can be presumed that ancient Hindus were quite familiar with lac and its uses.

 

Scientific study of lac started much later. In 1709 Father Tachard discovered the insect that produced lac. First of all Kerr (1782) gave the name Coccus lacca which was also agreed by Ratzeburi (1833) and Carter (1861). Later Green (1922) and Chatterjee (1915) called the ac- insect as Tachardia lacca (kerr). Finally, the name was given as Laccifer lacca.

 

In India, lac is cultivated in Jharkhand, Chhattisgarh and parts of Madhya Pradesh, Maharashtra and West Bengal primarily using Kerria lacca (Kerr).

 

Systematic Position:

A number of species of lac insects are known, of this Laccifer lacca is by far the most important and produces the bulk of the lac for commerce. It belongs to—

Phylum — Arthropoda                                               

Class —   Insecta 

Order Hemiptera

Super-family — Coccidae

Family — Lacciferidae

Genus Laccifer 

Species — lacca



Morphology- 

Lac insects are dimorphic i.e. male and female insets iffer in shape, size, and in presence or absence of some body parts.

 

Male Lac-insect:

 

The adult male is red in colour and smaller in size than the female insect. The length is about 1.2-2.5 mm. The body is typically divided into head, thorax and abdomen. Head bears a pair of eyes and seven segmented antennae. Thorax has 3 pairs of legs. The male lac insect may be either winged with one pair of hyaline wing on its thorax or wingless. Mouth parts are inactive in adults so a male adult insect is unable to feed. The adult male insect has a life span of 3 to 4 days, don’t eat and die after copulating with females. The eight-segmented abdomen ends into a short chitinous prominent sheath containing penis. A pair of white elongated caudal seta or filament is present on either side of this sheath.

Female lac-insect:

The female lac insect has a pyriform body measuring about 4-5 mm in length. The body is indistinctly divided into head, thorax and abdomen. Head bears a pair of degenerated antennae. Eyes are absent. Mouthparts are of piercing and sucking type. Posterior to mouth lie a pair of spiracles which ensures smooth breathing of the insect during lac secretion. Thorax has degenerated legs and lack wings. At the posterior part of the body, a triangular area – the anal tubercle encircling some setae is present. Near to it are present 2 branchial openings and one small chitinous spine called dorsal spine. The female larvae after settling down once never move again and thus these parts become useless and ultimately atrophy. It is female lac insect which secretes the bulk of lac for commerce.


Lac-insect

LIFE CYCLE:

Lac insect is a minute crawling scale insect which inserts its suctorial proboscis

into plant tissue, sucks juices, grows and secretes resinous lac from the body. Its own body

ultimately gets covered with lac in the so called CELL’. Lac is secreted by insects for protection from predators. The cell produced by male and female differ in shape, and can be easily distinguished from each other.

Male cells are elongated and cigar shaped. There is a pair of branchial pores in the anterior side and a single large circular opening covered by the flap in the posterior side. It is through the posterior circular opening that the matured male lac insect emerges out of its cell.

Female cell is oval, having a pair of small branchial pores in anterior side and a single round anal tubular opening in posterior side. Through the anal tubular opening are protruding waxy white filaments, secreted by the glands in the insects body, which is an indication that the insect inside the cell is alive and is in healthy condition. These filaments also prevent the blocking of the pore during excess secretion of lac.

 

Different forms of lac cell


Fertilization:

          After attaining the maturity, males emerge out from their cells and walk over the lac incrustations. The male enters the female cell through anal tubular opening and inside female cell it fertilizes the female. After copulation, the male dies. One male is capable of fertilizing several females. Females develop very rapidly after fertilization. the female insects and of theenveloping lac cells increase at a faster rate than the earlier stage and reaches a size several times more than that of the male lac cell.

The Life cycle of lac insect takes about six months and consists of following stages: egg, nymph instars, pupa (Pseudo) and adult.

The life of lac insects begins with the completion of embryonic development within the body of the mother when eggs change their position in the ovariole. Eggs then travel through oviducts and come out of the mother into the specially built “incubation chamber” formed by making sufficient gap between the body and resinous cell. Hatching of eggs mostly takes place after reaching the incubation chamber and is called as ovo-viviparity.

Eggs - Female lays 200-500 ready to hatch eggs, i.e. the embryos are already fully developed in eggs when these are laid. Eggs hatch within a few hours of laying, and a crimson-red first instar nymph called crawlers come out.

Nymph (larva)- The crawler measures 0.6 x .25 mm in size. The emergence and mass movement of nymph from female cell to the new off-shoots of host plant is called swarming, and it may continue for 5 weeks. The nymphs crawl about on branches. On reaching soft succulent twigs, the nymphs settle down close together at rate of 200-300 insects per square inch. At this stage, both male and female nymphs live on the sap of the trees. They insert their suctorial proboscis into plant tissue and suck the sap. After a day or so of settling, the nymphs start secreting resin from the glands distributed under the cuticle throughout the body, except mouth parts, breathing spiracles and anus. The resin secreted is semi-solid which hardens on exposure to air into a protective covering. The nymphs molt thrice inside the cells before reaching maturity. The duration of each instar is dependent on several factors, viz. temperature, humidity and host plant.

Adult -After about 6-8 weeks the larva metamorphoses into adults. Only the male undergoes complete metamorphosis, it loses its proboscis, develops antennae, legs and a pair of wings. The females undergo incomplete metamorphosis. Females retain her mouth parts but fail to develop any wings, eyes or appendages. Female becomes an immobile organism with little resemblance to an insect. The sex can be determined even during the early stages of development. As in case of males the growth is more on the longitudinal axis and in females the growth is more in vertical axis. The life span of the female is longer than that of the males. Now the male emerges out from its cell, moves on lac incrustation and enters the female cell for fertilization. In this way the life cycle is completed.


Life cycle of lac insect


CULTIVATION OF LAC (LAC CULTURE):

Cultivation of lac involves proper care of host plants, regular pruning of host plant, infection or inoculation, crop-reaping, control of insect pests, and forecast of swarming, collection and processing of lac.

Host Plants:

Lac insects thrive on twigs of certain plant species, suck the plant sap, and grow all the while secreting lac resin from their bodies. These plants are called host plants. Followings are very common host plants in India.


    1.    Butea monosperma (Vern. Palas)

2.    Zizyphus spp (vern. Ber)

3.    Schleichera oleosa (Vern. Kusum)

4.    Acacia catechu (Vern. Khair)

5.    Acacia arabica (Vern. Babul)

6.    Grewia teliaefolia (Vern. Dhaman preferred in Assam)

7.    Ficus benghalensis (Vern. Bargad)

8.    Ficus religiosa (Vern. Peepal)

9.    Arhar (Cajanus indicus)

 

Care of host plants -The first and perhaps the most important prerequisite for cultivation of lac is the proper care of the host plant. It is the host plants on which lac insects depend for their food, shelter and for completion of their life cycle. There are two ways for the cultivation of host plants. One is that plants should be allowed to grow in their natural way and the function of lac- culturist is only to protect and care for the proper growth of plants.

Another way is that a particular piece of land is taken for the purpose and systematic plantation of host plant is made there. Regular watch is necessary in this case by providing artificial manures, irrigation facilities, ploughing and protecting the plants from cattle and human beings for which the land should be fenced. The larvae of lac insects are inoculated on host plants only after the host plants have reached a proper height.

Pruning of host plant -The lac larvae feed on the cell sap by inserting their proboscis in the tender twigs. The proboscis can only be inserted in the tender young off-shoots. For this before inoculation, prunning of lac host plants is necessary. The branches less than an inch in diametre are selected for pruning. Branches half inch of less in diametre should be cut from the very base of their origin. But the branches more than half inch diametre should be cut at a distance of 1 ½ inch from the base.


Propagation of Lac Insects (Inoculation) -

Propagation means the spread of lac insects on the same or different host plants. This is done by inoculation of newly hatched (Brood) nymphs. The method by which the lac insects are introduced to the new lac host plant is known as inoculation. This may be of two types, namely “Natural infection” and “Artificial infection”. When infection from one plant to other occurs by natural movements of insect, it is called natural infection. This may be due to overcrowding of insect population and nonavailability of tender shoots on a particular tree.

Artificial infection takes places through the agencies other than those of nature. Prior to about two weeks of hatching, lac bearing sticks are cut to the size of six inches. They are called Brood lac”. Brood lacs are then kept for about two weeks in some cool place.

Three different ways of artificial inoculation of lac

When the larvae start emerging from this brood lac, they are supposed 
to be ready for inoculation. Strings со be used for tieding the brood lac with the host plant may be of different types in longitude infection the brood lac is tied in close contact with host branches. In lateral infection the brood lac is tied across the gaps between two branches. In interlaced method, brood lac is tied among the branches of several new shoots.

LAC CROP:

The lac insects repeat its life cycle twice in a year. There are actually four lac crops since the lac insects behave in two ways either they develop on Kusum plants or devlop on plants other than Kusum. The lac which grows on Non-Kusum plants is called as “Ranjeem lac,” and which grows on Kusum plant is called as “Kusumi lac. Four lac crops have been named after four Hindi months in which they are cut from the tree. They are as follows:

Ranjeeni Crop:

(i)    Katki: Lac larvae are inoculated in June-July. Male insect emerges m August-September. Female give rise to swarming larvae in October-November and the crop is reaped in Kartik (October and November).

(ii)   BaisakhiLarvae produced by Katki crop are inoculated in October-November, male insects emerges in February-March, females give rise to swarming larvae m June-July, the crop is reaped in Baisakh (April-May).


Kusumi Crop:

(i)   Aghani:

Lac larvae are inoculated in June-July, male insect emerges m September, female give rise to swarming larvae in January-February and crop is reaped in Aghan (December-January).


(ii)   Jethoi:

The larvae produced by Aghani crop is inoculated in the month of January- February, male emerges in March-April, female give rise to swarming larvae in June- July and the crop is reaped in the month of Jeath (June-July).

The time of infection with swarming larvae, the time of emergence of male insects, the time of reaping the crop, and the time of producing swarming larvae by female etc., are shown m tabular form below -

Lac Crop

HARVESTING OF LAC:

Harvesting is the process of collection of lac from host plants. It is done by cutting the lac encrusted twigs when is crop is mature. It may be of two types:-

a)    Immature harvesting In Immature harvesting, lac is collected before swarming, and lac thus obtained is known as ‘ARI LAC’. The immature harvesting has drawbacks, as the lac insects may be damaged at the time of harvesting. However, in case of palas lac (Rangeeni lac), it is found that ARI Lac gives better production. Hence, ARI lac harvesting is recommended in case of palas only.

b)   Mature harvesting -In mature harvesting, lac is collected after swarming. The lac obtained is known as mature Lac. To know the exact date emergence and swarming of nymph, a simple visual method is adopted. A yellow spot develops on the posterior side of lac cell towards crop maturity. This spot spread forwards until it covers half of the cell. Cutting of twigs for harvest can be done at any time between the stages while yellow spot occupies one third to one half of the cell area. It is sometimes desirable to wait till the emergence of the first few nymphs. The harvesting periods of different crops are different. The kartiki crop is harvested in Oct. /Nov.; baisakhi, in May/June; aghani in Jan/Feb.; and jethwi, in June/July. When the nymphs have escaped from the brood lac, what is left is the stick lac or phunki lac. These sticks should be tied in bundles and immersed in water, preferably running water for 3- 4 days, keeping them well under waters with help of heavy stones. The stick lac should then be kept in shade for drying. 

Scraping and Processing of lac:

Lac encrustations are removed from the twigs of host plant by scraping. after which they should not be exposed to sun. Various forms of lac are obtained at different stages of their purification. These are-

1.   Stick lac -The raw lac thus obtained is known as raw or crude lac or scraped lac or stick lac. This crude lack consists of resin, encrusted insect body, lac dye, sand and twig debris. The freshly scraped lac contains a lot of moisture and usually left to dry. The quality and value of stick lac depend very much upon variety of factors, viz. host tree, climate, whether the crop is harvested before or after emergence of larvae, and the method of drying and storage. The stick lac can not be stored for longer duration, as the lac has high moisture content and therefore tendency to form lump, and become low quality.


2.   Seed lac -The scraped lac is grinded in hard stone mills. The unnecessary materials are sorted out In order to remove the finer particles of dirt and colour, this lac is washed repeatedly with cold water. Now at this stage it is called as “Seed lac” and is exposed to sun for drying.

3.    Shellac-The shellac is the finished and purified product and is commonly used across the world. Seed lac is processed into shellac by any of the three methods: hand made country Process or heat process or solvent process. In melted process the seed lac is melted by steam heat. The molten soft lac is squeezed through filter by means of hydraulic pressure. The filtered molten lac is drawn into long and continuous sheets with help of roller. The sheet is then broken into pieces called flakes. Colour or different chemicals may be mixed during melting process for particular need. Commercially available shellac are Orange shellac, Lemon one shellac, Orange fine shellac etc.

COMPOSITION OF LAC:

The major constituent of lac is the resin. Lac resin is a polyester complex of straight- chain hydroxy fatty acids of C14 – C18 carbon chain (such as Aleuritic acid, butolic acids), mono- and dihydroxy acids along with hydroxy terpenic acids. Other constituents present are : dye, wax, sugar, proteins, soluble salts, sand, woody matter, insect body debris etc. Lac wax is a mixture of anthroquinoid derivatives. . The approximate percentage of different constituents of lac is given below:

Resin 68 to 90%

Dye 2 to 10%

Wax – 5 to 6%

Mineral matter 3 to 7%

Albuminous matter 5 to 10%

Water 2 to 3%


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